Analysis Note

ControlHeLa Cells

Application

Research Sub CategoryHistones

Milli-Mark Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor™488 Antibody detects level of dimethyl-Histone H3 (Lys9)-Alexa Fluor™488 & has been published & validated for use in FC.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Epitope: Methylated at Lys9dimethylated Lys 9 of Human Histone H3

KLH-conjugated, 2X-branched synthetic peptide corresponding to dimethyl-lysine at residue 9 of human Histone H3.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Purified rabbit polyclonal IgG conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA

Quality

Evaluated by flow cytometry using HeLa cells

Specificity

Antibody recognizes human Histone H3 dimethylated on lysine 9.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.

Target description

15.5 kDa Calculated